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Objective@#To analyze the relationship between thrombin activatable fibrinolysis inhibitor ( TAFI ) and coronary heart disease ( CHD ), and to provide evidence for the prevention of CHD. @*Methods@#The patients with CHD in Fushun Central Hospital in Liaoning Province were selected as the case group, the patients without CHD in the same hospital and period were selected as the control group. The demographic information and clinical examination results ( serum TAFI, lipid, glucose, etc. ) were collected to analyze the association between TAFI and CHD by logistic regression models.The multivariate logistic regression analysis was used to explore the relationship between TAFI and CHD.@*Results@#There were 222 cases, including 100 cases of stable angina, 44 cases of unstable angina and 78 cases of acute myocardial infarction, and 222 controls. The median ages of cases and controls were 62 and 57 years old. The results of multivariate logistic regression analysis showed that serum TAFI>22.88 μg/mL ( P75 of controls ) was associated with the risk of CHD ( OR=1.619, 95%CI: 1.011-2.593 ), unstable angina ( OR=2.917, 95%CI: 1.433-5.939 ) and acute myocardial infarction ( OR=2.626, 95%CI: 1.007-6.847 ). @*Conclusion@#The high level of TAFI is related to CHD, unstable angina and acute myocardial infarction.
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Objective@#To explore the mechanical changes and distributions of pressure in each characteristic intervertebral disc after short segment unilateral pedicle screw fixation in Chinese mini-pigs.@*Methods@#Six characteristic spine segments of 6-week-old Chinese mini-pigs (T3-4、T11-12、T15-L1、L3-4、L4-5 segments with adjacent upper and lower vertebrae) were chosen as experimental models. A biomechanical machine and pressure sensitive film were used to measure the pressure on the vertebral columns when they were subjected to pressures of 200 N as unfixed group. Compression was carried out under vertical, 5 °flexion, 5 °extension and 5 °lateral bending, respectively. Then the PCF (posterior cervical fixation) system were fixed on all segments (left side) as fixed group, and biomechanical tests were repeated to obtain data. A Fujitsu densitometer (Japan) was used to measure the pressure on each direction of the pressure sensitive film.@*Results@#In vertical pressures, 5° flexion and 5°extension conditions, the posterior pressure of T3-4、T11-12、T15-L1、L3-4 and L4-5 intervertebral discs were decreased after fixation, the differences were statistically significant; The fixed side pressures of T3-4、T11-12、T15-L1、L3-4 and L4-5 in the fixed group were significantly lower than those in the unfixed group in the corresponding segments and load conditions, and the differences were statistically significant. The concave disc pressures of T3-4、T11-12、T15-L1、L3-4 and L4-5 in the fixed group were 0.83±0.12, 0.93±0.12, 0.50±0.05, 0.60±0.10 and 0.55±0.08 MPa respectively, which were lower than those of the unfixed group 1.70±0.18, 1.79±0.14, 1.84±0.22, 1.74±0.28, 1.60±0.23 MPa, and the differences were statistically significant. The total disc pressure of the fixed group was significantly lower than that of the non-fixed group in vertical compression, 5 degree flexion and 5 degree extension, and the differences were statistically significant.@*Conclusion@#Unilateral pedicle screw fixation can decrease the pressure on fixed side of fixed group in all conditions and change the distribution of pressure. The posterior pressure decreased particularly. Short- segment unilateral pedicle screw fixation can decrease the average pressure of intervertebral disc, which is an excellent surgery in curing scoliosis in young children.
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Objective@#To explore the international development trends and research hotspots of outdoor activities affecting the progression of children’s myopia, and to provide a reference for researching on effective ways to prevent children’s myopia.@*Methods@#Totally 291 relevant documents included in the "Web of Science" core set database were used as research objects, and CiteSpace software was used for visual analysis.@*Results@#At present, the publications in this field were mainly in the United States(81), China(80), Australia(76), and Singapore(33); the top three research institutions were "Natl Univ Singapore"(29), "Australian Natl Univ"(27), "Capital Med Univ"(25); the main authors were "Saw SM", "Morgan IG", "Mitchell P". The field has been developed on the basis of "Ophthalmology", "Public, Environmental and Occupational Health", and has been integrated into 32 disciplines. The research content included "exploration of high risk factors for the progression of children’s myopia" and "outdoor activities", "intervention in children’s progression of myopia" and "longitudinal tracking of children’s vision development". Randomized clinical trials that longitudinally track the correlation between changes in eyeballs and the progression of myopia and the effects of outdoor activities on the biological characteristics of children’s eyeballs have become a hot topic in this field.@*Conclusion@#Research on the effects of outdoor activities on the progression of myopia in children has increased dramatically. The study of increasing outdoor activities to interfere with the progression of myopia in children and the vertical tracking of key factors affecting the biological characteristics of children’s eyeballs have become the current international trends.
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Objective To explore the mechanical changes and distributions of pressure in each characteristic interverte?bral disc after short segment unilateral pedicle screw fixation in Chinese mini?pigs. Methods Six characteristic spine segments of 6?week?old Chinese mini?pigs (T3?4、T11?12、T15?L1、L3?4、L4?5 segments with adjacent upper and lower vertebrae) were cho?sen as experimental models. A biomechanical machine and pressure sensitive film were used to measure the pressure on the verte?bral columns when they were subjected to pressures of 200 N as unfixed group. Compression was carried out under vertical, 5°flex?ion, 5°extension and 5°lateral bending, respectively. Then the PCF (posterior cervical fixation) system were fixed on all segments (left side) as fixed group , and biomechanical tests were repeated to obtain data. A Fujitsu densitometer (Japan) was used to mea?sure the pressure on each direction of the pressure sensitive film. Results In vertical pressures, 5°flexion and 5°extension condi?tions,the posterior pressure of T3?4、T11?12、T15?L1、L3?4 and L4?5 intervertebral discs were decreased after fixation,the differ?ences were statistically significant;The fixed side pressures of T3?4、T11?12、T15?L1、L3?4 and L4?5 in the fixed group were signif?icantly lower than those in the unfixed group in the corresponding segments and load conditions, and the differences were statisti?cally significant. The concave disc pressures of T3?4、T11?12、T15?L1、L3?4 and L4?5 in the fixed group were 0.83±0.12, 0.93± 0.12, 0.50±0.05, 0.60±0.10 and 0.55±0.08 MPa respectively, which were lower than those of the unfixed group 1.70±0.18, 1.79± 0.14, 1.84±0.22, 1.74±0.28, 1.60±0.23 MPa, and the differences were statistically significant. The total disc pressure of the fixed group was significantly lower than that of the non?fixed group in vertical compression, 5 degree flexion and 5 degree extension, and the differences were statistically significant. Conclusion Unilateral pedicle screw fixation can decrease the pressure on fixed side of fixed group in all conditions and change the distribution of pressure. The posterior pressure decreased particularly. Short? segment unilateral pedicle screw fixation can decrease the average pressure of intervertebral disc, which is an excellent surgery in curing scoliosis in young children.
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Objective To analyze the clinical characteristics of Brucellar spondylitis and evaluate its surgical treatment effect.Methods From 2002 to 2015,the clinical data of diagnosed patients with Brucellar spondylitis aged ≥65 years old were collected retrospectively in the First Affiliated Hospital of Hebei North University,patients' clinical manifestations and characteristics,laboratory diagnosis,imaging changes and surgical treatment effects were analyzed.Pain and imaging scores and clinical efficacy were evaluated according to follow-up data within 12 months after surgery.Results A total of 38 cases of senile patients with Brucellar spondylitis were studied.The main clinical symptoms during the visit were persistent severe lumbar back pain,local tenderness,obvious percussion pain,muscle spasm,and constrained movements of spine.Rose bengal plate test (RBPT) was positive in 11 cases,serum tube agglutination test (SAT) titer was higher than 1:100 in 28 cases.During the operation,28 cases of patients with inflammatory granuloma or abscess were cultured positive for Brucella.Before surgery,manifestations of X-ray:of which 29 cases showed intervertebral space narrowing,the density increased,and the margins of the verbebral bodies destructed;of which 9 cases showed vertebral body bone sclerosing hyperplasia in the shape of a bird's beak,forming a bone bridge with the adjacent vertebral body margin.Manifestations of CT:of which 29 cases showed different sizes and multiple lesions of the vertebral body margin,and hyperplasia and sclerosis around the lesion,and destruction of the lesion in the new bone tissue to form "lacy vertebra";in 9 cases,the destruction of intervertebral discs showed isodensity shadow,and the hyperplasia and sclerosis of articular surface resulted in the formation of "labial" osteophytes;of which 10 cases showed vertebral body destroyed the plane in which the bilateral psoas were widened and abscess formed.Manifestations of MRI:of which 38 cases showed that the vertebral bodies,intervertebral discs,accompaniment and the intra of vertebral canal were uneven high signal and dural sac or cauda equina compression.The pain scores [(2.1 ± 0.2),(0.7 ± 0.4),(0.2 ± 0.1),(0.0 ± 0.0),(0.0 ± 0.0) scores] at 2 weeks and 1,3,6,12 months after surgery were significantly lower than that before surgery [(9.2 ± 0.3) scores,P ＜ 0.05].The blind imaging evaluation scores [(4.68 ± 0.04),(4.92 ± 0.08),(5.00 ±0.00) scores] at 3,6 and 12 months after surgery were significantly higher than that before surgery [(0.37 ± 0.03) scores,P ＜ 0.05].The cure rates of clinical efficacy [92.11％ (35/38),100.00％ (38/38)] at 6 and 12 months after surgery were significantly higher than that of 3 months after surgery [78.95％ (30/38),P ＜ 0.05)].Conclusions Senile Brucellar spondylitis has the typical imaging features,laboratory examination is helpful to early diagnosis.Surgery is much better to relieve the pain and stable spine function and accelerate rehabilitation.
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Objective@#To explore the apoptosis-inducing effect of the Chinese medicinal compound CFF-1 on prostate cancer cells and its related molecular mechanisms.@*METHODS@#Normal prostate WPMY-1 cells and prostate cancer LNCaP, CWR22Rv1, PC3 and DU145 cells were treated in dehydrated alcohol with CFF-1 at 0, 2, 5, or 10 mg/ml for 24 hours. Then the viability of the prostate cells was detected by morphological observation, MTT and CCK-8 assay, nuclear condensation and disruption measured by DAPI staining, the cell cycle and apoptosis calculated by flow cytometry, the activity of the PI3K/AKT/FOXO1 signaling pathway and the expressions of its downstream apoptosis- and cycle-related proteins determined by Western blot.@*RESULTS@#CFF-1 significantly arrested the cell cycle in the G1 phase, decreased the cell viability and increased the nuclear condensation and disruption in a dose-dependent manner, and elevated the apoptosis rate of prostate cancer cells. At the molecular level, CFF-1 dose-dependently reduced the activity of the PI3K/AKT signaling pathway and phosphorylation of the FOXO1 protein, increased the transcription activity of FOXO1, and eventually regulated the expressions of cell apoptosis- and cycle-related genes.@*CONCLUSIONS@#The Chinese medicinal compound CFF-1 can significantly inhibit the growth, arrest the cycle, and induce the apoptosis of prostate cancer cells by decreasing the activity of the PI3K/AKT/FOXO1 signaling pathway, which suggests its potential clinical application value in the treatment of prostate cancer.
Subject(s)
Humans , Male , Antineoplastic Agents, Phytogenic , Pharmacology , Apoptosis , Cell Cycle , Cell Division , Cell Line, Tumor , Cell Proliferation , Cell Survival , Drugs, Chinese Herbal , Pharmacology , Forkhead Box Protein O1 , Metabolism , Neoplasm Proteins , Metabolism , Phosphatidylinositol 3-Kinases , Metabolism , Phosphorylation , Prostatic Neoplasms , Drug Therapy , Metabolism , Pathology , Proto-Oncogene Proteins c-akt , Metabolism , Signal TransductionABSTRACT
OBJECTIVE: To analyze the pressure change and distribution of the intervertebral disc of upper thoracic spine in vertical pressure and 5° flexion, extension, or lateral bending.
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<p><b>OBJECTIVE</b>To investigate the non-high-density lipoprotein cholesterol (non-HDL-C) level and the prevalence rate of a high non-HDL-C level in children aged 9-11 years in the Mianyang Science City area in Sichuan Province, China.</p><p><b>METHODS</b>From September to October, 2015, a field investigation was performed for the students from three primary schools in the Mianyang Science City area by cluster sampling. Fasting venous blood was collected for blood lipid tests. The cut-off value of serum non-HDL-C level and prevalence rate of a high non-HDL-C level in children aged 9-11 years in this area were calculated, as well as the prevalence rate of a high non-HDL-C level in obese children.</p><p><b>RESULTS</b>In the children aged 9-11 years in this area, the cut-off value of non-HDL-C level was 3.67 mmol/L, and the prevalence rate of a high non-HDL-C level was 3.7% (22/589). Compared with the non-obese children, the obese children had a significantly higher serum non-HDL-C level (P<0.01) and a significantly higher prevalence rate of a high non-HDL-C level (10.0% vs 2.9%; P<0.01).</p><p><b>CONCLUSIONS</b>The cut-off value of serum non-HDL-C level in children aged 9-11 years in the Mianyang Science City area is established. Obesity is associated with an increased prevalence rate of a high non-HDL-C level in children aged 9-11 years.</p>
Subject(s)
Child , Female , Humans , Male , China , Cholesterol , Blood , Lipoproteins , Blood , Obesity , BloodABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expressions of nucleolin in invasive cervical squamous cell carcinoma, cervical intraepithelial neoplasms (CIN) and normal cervical epithelial tissues and explore the role of nucleolin in the carcinogenesis and progression of cervical squamous cell carcinoma.</p><p><b>METHODS</b>Fifty specimens of invasive cervical squamous cell carcinoma, 65 specimens of CIN, and 60 adjacent normal cervical epithelial tissue specimens were examined immunohistochemically for nucleolin expression. The correlation of nucleolin expression levels with histological grades of invasive cervical squamous cell carcinoma and CIN were analyzed.</p><p><b>RESULTS</b>The specimens of invasive cervical squamous cell carcinoma showed a significantly higher positivity rate for nucleolin expression than CIN and normal cervical epithelial tissues, and the rate in CIN tissues was significantly higher than that in normal cervical epithelial tissues (P<0.01). The expression level of nucleolin was significantly higher in invasive cervical squamous cell carcinoma than in CIN and normal cervical epithelia tissues, and higher in CIN than in normal cervical epithelia tissues, whose immunostaining scores were 7.6±0.3, 6.1±0.2, and 3.0±0.2, respectively (P<0.01). The mean nucleolin immunostaining score was significantly higher in poorly and moderately differentiated than in highly differentiated cervical squamous cell carcinoma (7.9 vs 7.1, P<0.01), and higher in high grade CIN than in low grade CIN tissues (6.0 vs 4.0, P<0.01).</p><p><b>CONCLUSIONS</b>Overexpression of nucleolin plays an important role during carcinogenesis of cervical squamous cell carcinoma and is positively correlated with tumor progression of CIN and cervical squamous cell carcinoma.</p>
Subject(s)
Female , Humans , Carcinogenesis , Carcinoma in Situ , Carcinoma, Squamous Cell , Metabolism , Pathology , Uterine Cervical Dysplasia , Metabolism , Pathology , Disease Progression , Phosphoproteins , Metabolism , RNA-Binding Proteins , Metabolism , Uterine Cervical Neoplasms , Metabolism , PathologyABSTRACT
<p><b>OBJECTIVE</b>To detect the cell-surface-expressed nucleolin and investigate its tumor suppressing effect on the growth of hepatocellular carcinoma cells.</p><p><b>METHODS</b>To detect cell-surface-expressed nucleolin in the hepatocellular carcinoma cells by immunofluorescence and flow cytometry. To down-regulate the nucleolin expression level in hepatocellular carcinoma cells by RNA interference. The tumor-suppressing effect of cell-surface nucleolin on hepatocellular carcinoma cells was assessed by MTT and transwell chamber assays.</p><p><b>RESULTS</b>Nucleolin was expressed in the nuclei, cytoplasm and on the cell surface of hepatocellular carcinoma cells. ShRNA markedly decreased the nucleolin expression level in the cytoplasm and on the cell surface (P < 0.01), but the nuclear nucleolin remained unchanged. After downregulation of cell-surface nucleolin, MTT assays showed that the cell growth rate of hepatocellular carcinoma cells in the shRNA interference group was significantly inhibited as compared with that in the control group (P < 0.01). The transwell chamber assay showed that the mean transmembrane cell number in the shRNA interference group was significantly lower than that in the control group.</p><p><b>CONCLUSION</b>The results of this study show that downregulation of cell-surface nucleolin expression inhibits the growth of hepatocellular carcinoma cells in vitro.</p>
Subject(s)
Humans , Carcinoma, Hepatocellular , Genetics , Metabolism , Pathology , Cell Line, Tumor , Cell Membrane , Metabolism , Cell Movement , Cell Nucleus , Metabolism , Cell Proliferation , Cytoplasm , Metabolism , Down-Regulation , Liver Neoplasms , Genetics , Metabolism , Pathology , Phosphoproteins , Metabolism , RNA Interference , RNA, Small Interfering , Pharmacology , RNA-Binding Proteins , MetabolismABSTRACT
<p><b>BACKGROUND</b>Myofibroblastic sarcoma was used to be a controversial neoplasm. This study investigated the clinicopathological features of 20 cases of myofibroblastic sarcoma arising in different locations.</p><p><b>METHODS</b>The paraffin-embedded tissue samples from 20 cases of patients with myofibroblastic sarcoma were stained immunohistochemically, and 5 cases examined by electron microscopy. Student's t test was used to analyze the difference of Ki-67 labeling index between grade 1 and grade 2 myofibroblastic sarcomas.</p><p><b>RESULTS</b>Histologically, the tumors were composed of slender spindle cells with eosinophilic cytoplasm, and fusiform, tapering, wavy, or plump ovoid; vesicular nuclei and a small central eosinophilic nucleoli. Immunohistochemically, the tumor cells expressed smooth muscle actin (18/20), muscle specific actin (16/20), fibronectin (20/20) and desmin (2/20). Ultrastructurally, the tumor cells revealed abundant rough endoplasmic reticulum and longitudinally arranged fine filaments with focal densities in the cytoplasm. A clinical follow-up of 19 patients showed that 2 cases experienced local recurrence and distant metastasis 6 months to 4 years after the initial operation. Nine cases recurred locally 17 to 46 months after the initial excision, and 9 cases were alive with no evidence of disease.</p><p><b>CONCLUSIONS</b>Myofibroblastic sarcomas, which exhibit diverse histological appearance, can easily be misdiagnosed as benign tumors. Myofibroblastic sarcomas are local destructive lesions with frequent recurrence, and may metastase distantly.</p>